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Then phase2 is added to phasel under stirring

While diagnosis of DCM is based on clinical examination, imaging evidence of spinal cord compression or cord tethering on MRI is required to confirm the diagnosis (Table 2)

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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)