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The viability was measured by using Tecan Spark 10 microplate reader at 450 nm according to the manufacturers instructions
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Saben J, Lindsey F, Zhong Y, Thakali K, Badger TM, Andres A, et al
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The rapid ascorbate loss in extended dark occurs along with the induction of a range of carbohydrate starvation responses that are induced by this treatment [94]
