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doi: 10.3389/fnbeh.2022.844916 Received 29 December 2021 Accepted 27 April 2022 Published 23 May 2022 Volume 16 - 2022 Edited by Matthew J
In the loading reaction, the UB-carrying enzyme (UBE2L3) is charged by UBA1, and quenched by apyrase, which prevents further ubiquitin activation once UBE2L3 is discharged
GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column
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