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The next day, the serum-containing media was removed from each well and cells were washed with PBS before treating the cells in serum-free DMEM and 0.5% DMSO for 48 h

The human tri-peptide GHK and tissue remodeling

Hyperhomocysteinemia: an independent risk factor or a simple marker of vascular disease

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In addition, blood samples were also collected from patients on days 0, 1, 2, 3, 4, 8 and 15 of the first cycle and days 0, 8 and 15 of the second cycle for measurement of TdR, dUrd, Hcy and MMA concentrations
