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Description
Following incubation with each eryptosis inducer, phenotypic hallmarks of eryptosis were measured, namely PS exposure, cell size, intracellular calcium levels and hemolysis
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Furthermore, glutathione is involved in the metabolism of iron and the protection of red blood cells
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The cells were then harvested and lysed with B-PER containing 5mM imidazole, 1X cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (Roche)
