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Increase in Mrp1 expression and 4-hydroxy-2-nonenal adduction in heart tissue of Adriamycin-treated C57BL/6 mice

Julien, J

Customized luminex kits were purchased from Millipore Sigma (Catalog# HYCTOMAG-60K) and adapted to a 384 well plate format based on previously described methods ( Results Analytical Characterization of Antibody Aggregates Upstream processing material from three different stages of the purification process for the monoclonal antibody mAb1 (described further in the Materials and Methods section) were utilized in this study: material from a 0.2 m filtered, neutralization/viral inactivation step (FNVI) that occurred after a protein A chromatography step, mAb1 material following an anion exchange chromatography step (AEX), and mAb1 material following a virus filtration step (VF)

The cytoplasmic calcium-activated calpain cleaves and activates caspase-12 in response to ER-released calcium (Tan et al., 2018)

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