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Following transient and stable expression in Nicotiana and Arabidopsis, respectively, the GSTs were recovered using Strep-Tactin affinity chromatography and the bound ligands desorbed and characterized by LC-MS

The soluble fraction was separated from debris by spinning down at 20,000 g for 20 min

Bacterial cells were imaged live using microscope (63 glycerol-immersion objective) with a fast lifetime contrast (FALCON) module

Peroxynitrite is considered the ultimate oxidant responsible for the mitochondrial membrane permeability transition pore (MPTP) opening, potentially leading to liver cell death [50-52]

Subramaniam D