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Data processing and normalizations Proper data cleansing/de-noising and normalizations of omics data are important processes in biomarker identifications

Resuspended samples were loaded onto equilibrated spin columns (2000 g , 2 min), passing the entire sample twice through the column

Tsang MLS, Schiff JA (1975) Studies of sulfate utilization by alage

After washing with TBS containing 0.01% Tween 20 (TBST) three times, membranes were then incubated with the primary antibodies overnight at 4 C

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