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They should be only used in analytical applications if the ligand is quite stable or in preparative applications if both the ligand and target are relatively stable and it is enable to recover their activity after such elution [8,23]
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In the article below, I introduce nuances and confounding factors, but in my opinion, the paradox remains unresolved
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QL: Writing original draft
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Statistical significance was calculated by one-way ANOVA in a and two-way ANOVA followed by Dunnetts multiple comparisons test with Bmal1-KO mice set as the reference group in b
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