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Development of an assay pipeline for the discovery of novel small molecule inhibitors of human glutathione peroxidases GPX1 and GPX4

The most important function of vitamin D is in the regulation of Ca 2+ concentration in circulating blood, which deficiency may lead to diseases such as rickets in children and osteomalacia in adults

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To determine the kinetic constants for ubiquinone-Q1, DCPIP was omitted and the concentration of the quinone was varied between 0 mM and 0.06 mM with a concentration of 0.6 g for either Saci_2032 or Saci_1118, while maintaining a fixed G3P concentration of 0.4 mM