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Description
Cell pellets were resuspended in ice-cold lysis buffer (20mM HEPES-KOH pH 7.4, 150 mM NaCl, 0.1% Triton X-100, 10% glycerol, 5mM DTT) supplemented with protease inhibitors (1 mM AEBSF, 0.8 mM Aprotinin, 15 mM E-64, 10 mg/mL Bestatin, 10 mg/mL Pepstatin A and 10 mg/mL Leu-peptin), incubated on ice for 15 min and spun down at 16,000 x g for 15 min at 4C
Wajih N, Sane DC, Hutson SM, Wallin R
Avocado has a mild taste that pairs well with most smoothie ingredients
Front Oncol 7:193
Aprile, M., Costa, V., Cimmino, A
