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After another round of washing with the wash buffer, the eluted protein was subjected to immunoblotting analysis using HRP-conjugated rabbit polyclonal anti-GST (1006-3, Huabio, dilution 1:500) or mouse monoclonal anti-His (66005-I-Ig, Proteintech, dilution 1:1000) antibodies, respectively

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Challenges include batch-to-batch variability, absence of standardized extraction protocols, and inconsistent concentrations of bioactive constituents, all of which complicate compliance with Good Manufacturing Practice (GMP) standards and hinder reproducibility in clinical trials

Appl Microbiol Biotechnol 103:16811691

Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway