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After washes in PBS with 0.1% Tween (PBS-T) for 30 min (10 min per wash), a secondary antibody was applied for 1 h at room temperature in the blocking buffer (see Supplementary Table 4 for primary and secondary antibodies used in this study)

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Cytokines derived from M1 macrophages impair tubular autophagy, exacerbating inflammatory injury, whereas M2-mediated fibrosis disrupts vascular architecture, creating hypoxic niches that further drive M2 polarization (55, 56)

Unfortunately many doctors do not take into account neurological symptoms and the patient is automatically placed onto a three monthly maintenance dose of B12 injections after loading doses