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The clinical and prognostic correlation of HRNPM and SLC1A5 in pathogenesis and prognosis in epithelial ovarian cancer

[DOI] [PubMed] [Google Scholar] 36.Chapple IL, Mason GI, Garner I, Matthews JB, Thorpe GH, Maxwell SR, et al

In this technique, a specific ligand or binding partner (for example, an enzyme inhibitor or a specific antibody) of the protein of interest (POI) is covalently immobilized onto a stationary phase, usually a biocompatible hydrophilic polymer such as agarose

Cells were lysed by the direct addition of sample buffer and protein lysates separated on 10% or 16% Tris-Glycine mini gels by SDS-PAGE, before transfer onto nitrocellulose membranes

AONs are readily available through solid-phase oligonucleotide synthesis and therefore represent an ideal starting point for most oligonucleotide conjugations
