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used the Apc Min /+ mouse model and induced inflammation-driven tumor formation in them (45)

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After resolving samples on SDS-PAGE and nitrocellulose membrane transfer, western blot normalization was performed with the Licor Revert 700 Total Protein Stain Kit as described in their protocol
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IGF-1 LR3 typically comes as a lyophilized powder and needs to be reconstituted with sterile or bacteriostatic water
