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To test this, we used transgenic animals expressing the transcriptional rab-11.2 p::YFP reporter, which serves as an effective fluorescent diagnostic of NHR-49 activation, though seemingly not a direct transcriptional target 23,42

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12( S )-HEPE promotes glucose uptake by triggering G s PCR, which leads to PI3K-mTORC2-AKT activation and GLUT4 translocation to the plasma membrane and utilization in BAT, thereby improving glucose tolerance and insulin sensitivity in diet-induced obese (DIO) mice 119

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