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2.9 ROS and mtROS detection 2,7-Dichlorofluorescin diacetate (DCFH-DA, Beyotime, China) was used to measure total intracellular ROS levels

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However, to date, it is unknown which molecules are required to direct sortilin itself into the regulated secretory pathway, with previous studies demonstrating that sortilin can sort cargo to the constitutive pathway, the regulated secretory pathway, and the endosomal pathway [9, 57, 58]