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Description
n = 6 CuR+Vehicle, 5 CuD+Vehicle, 16 CuR+MTX, and 17 CuD+MTX mice from weeks 16
Animals in control group received 2 mls of normal saline per day
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GSHc levels were not influenced by substance use ( U 175 = 0.197, p = 0.84) or physical activity ( U 175 = 0.99, p = 0.31)
Assay of mitochondrial membrane potential and glucose uptake For evaluation of mitochondrial transmembrane potential cells were incubated with Mitotracker Red (Life Technologies Ltd) at concentration of 200 nM (30 min, 37 C) prior to cytofluorimetric analysis
