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This activation, in turn, upregulates the vascular endothelial growth factor and promotes angiogenesis (102)
In the case of naloxone, which has a relatively short duration of action, severe withdrawal usually lasts less than an hour with symptoms typically persisting no more than 90 min [Citation2527]
Then, 20 L of Luciferin Detection Reagent was added per well and incubated for 30 min
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