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A later study using lineage-specific fluorescent reporters did not support tumor cells as a source of endothelial cells in glioma, instead demonstrating that glioma cancer stem cells can differentiate into pericytes and that specific depletion of pericytes disrupted tumor vessels and tumor growth [81]

Furthermore, vitamin C decreases HIF-1 level in TC cells, thus downregulating GLUT1 expression [218]

Cells in exponential phase were supplemented with 100m -Glu-met, metabolite quenching and cell harvesting were done at different time intervals i.e