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Description
We hypothesized that increasing the antioxidant capacity of the brain would facilitate sleep-dependent decreases in oxidative stress, decreasing the time required to dissipate sleep needs during NREMS

10.1038/ncomms2621 94 KrappA.DavidL

Increased oxidative stress suggested by low serum vitamin e concentrations in patients with chronic fatigue syndrome

DMSO:H 2 O 0.01% DMSO Step 2 an additional 100 L of media containing 3 M SYTOX and 0.2% Triton X-100 was added in order to permeabilize all cells and incubated for 1 h before fluorescence values measured (Step 2)

References Butchko HH, Stargel WW
