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MitoTracker Red CMXRos Staining To locate the mitochondria, cultured cells were stained with 200 nM MitoTracker Red CMXRos (Thermo Fisher Scientific, USA) for 15 min at room temperature in the dark
For the sequential samples from the first 34 patients (172 paired samples), DNA was isolated from both CTC and plasma samples obtained from ficoll preparations (Supplementary Figure 2)
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