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Description
After blocking with 5% milk in TBST buffer, the membrane was incubated with primary antibodies followed by appropriate horseradish peroxidase-conjugated secondary antibodies (listed in Supplementary Table 1)
The standard dosage for severe deficiency typically starts with 1 mg daily injections, transitioning to weekly and then monthly maintenance doses
When a provider wants to support certain nutrient pathways or when B12 status is being evaluated, lipotropics may be discussed

10.1111/j.1749-6632.1987.tb48757.x

How often should I get intramuscular injections