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Pharmacol Ther 244:108373 da Cunha Germano BC, de Morais LCC, Idalina Neta F, Fernandes ACL, Pinheiro FI, do Rego ACM et al (2023) Vitamin E and its molecular effects in experimental models of neurodegenerative diseases

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Current evidence suggests that FRDA pathogenesis creates a highly conducive environment for lipid peroxidation (Figure 1), driven by dysregulated iron handling, amino acid metabolic shifts, and the failure of both glutathione (GSH)-dependent and independent pathways [29,63]

Biochem., 2009, 103 (3), 333341

Pellets were resuspended in lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5% (v/v) glycerol, 15 mM imidazole, 4 mM BME and 1 tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001)), with 1 ml of lysis buffer per 240 ml of overnight culture