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[DOI] [PubMed] [Google Scholar] 202.Zafrilla P., Mulero J., Xandri M.J., Santo E., Caravaca G., Morillas M.J

Journal of Pharmacy and Bioallied Sciences;3 (3):397-402

Following a three-day incubation, DNA content was quantified using Picogreen (Life Technologies), as previously described ( 50 ) were determined from nonlinear regression of three biological replicates (GraphPad Prism)

Also known as Mary thistle or holy thistle, milk thistle grows in Mediterranean countries and people use it to make natural remedies

The brain, being highly dependent on glucose and oxygen delivered via cerebral blood flow, becomes especially vulnerable when cerebral blood flow declines due to aging or sleep deprivation, resulting in metabolic instability and overproduction of ROS (Leithner and Royl, 2014