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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

Cell lineage-based stratification for glioblastoma

doi: 10.1097/MD.0000000000036144 199 SieperJPoddubnyyD

The loss of induction of the -glutamyl cysteine synthase-light subunit (GCS-LS) is considered to be the responsible factor for loss of liver GSH level even under ethanol treatment [70]
