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It is usually due to the cystic duct being blocked, causing a backup of bile fluids that give ordinary stool its normal color

2.5 Methodological quality assessment The methodological quality of each included systematic review was independently assessed by two reviewers using the AMSTAR-2 (A MeaSurement Tool to Assess systematic Reviews 2) tool

The membrane was washed with Tris-buffered saline (TBS), containing 0.1% Tween 20 (Beyotime Institute of Biotechnology) three times and was subsequently incubated with horesradish peroxidase-labeled secondary antibody (1:2,000

Studies suggest 10-20 times higher tissue concentrations compared to topical application of equivalent amounts

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