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Secondary antibodies were used at a dilution of 1:10,000

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Although the number of species detected in the sample increased (increased species richness), some species may over proliferate, leading these species to dominate the community, thus reducing evenness (the Simpson index decreased)

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The deficiency or mutation of Kelch-like ECH-associated protein 1 (KEAP1), mediated by p62, leads to the activation and upregulation of Nrf2
