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Collapse assay Cos7 cells were plated (1 10 5 cells/ml) onto glass coverslips, and 24 h later, cells were transiently transfected with plasmids encoding for empty vector (pmCherry) or co-transfected with myc-PlexinB1 together with pmCherry-RanWT, pmCherry-RanQ69L, pmCherry-RanT24N or pmCherry using Lipofectamine 2000 as per manufacturers instruction

Molecular cloning and characterization of -glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694

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