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In some cases NEM was added to the deproteinizing solution [24,43]

The antibody according to any one of claims 2-6, wherein said cells that express or overexpress are cells of a cancer selected from the group consisting of mesothelioma, testicular cancer, endometrial cancer, and subsets of ovarian, pancreatic, and non small cell lung cancers

Once reconstituted, solutions should be stored under refrigeration and used within 24 to 48 hours to minimize degradation

Further, biomarker concentrations are tricky to interpret: Total GSH concentration often fails to accurately reflect redox balance, which is better indicated by the GSH to GSSG ratio, plasma/serum concentrations are often a weak correlate of tissue level (the locus of action), and compartmentalization within cells (cytosol versus mitochondria) adds another layer of complexity often out of grasp in clinical samples [31]