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Routine monitoring and supplementation may be appropriate this is a clinical conversation to have with a prescribing physician

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Ni-NTA purification was performed, and the buffer was exchanged with 50 mM Tris-HCl, pH 6.0, using a PD-10 desalting column with Sephadex G-25 resin (GE Healthcare)

[DOI] [PMC free article] [PubMed] [Google Scholar] 30.Ferdinand KC, White WB, Calhoun DA, Lonn EM, Sager PT, Brunelle R, Jiang HH, Threlkeld RJ, Robertson KE, Geiger MJ