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Pain, a complex symptom encompassing both sensory and emotional dimensions, constitutes a significant global public health issue

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Ulcerative colitis (UC) is the most common chronic inflammatory disease of the intestinal tract in clinical practice, and long-term chronic inflammation leads to repeated damage to and repair of the colonic mucosa, which may progress to malignancy through atypical hyperplasia

Figure 2. Retained His-tagged protein binding capacity in presence of EDTA. Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280

Learn what heat exhaustion does to your body, how it differs from dehydration and heatstroke, and what to do if symptoms develop