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This document provides a brief overview of the cultivation techniques, while a separate comprehensive review (references [654,655,656,657]) can be referred to for a detailed description of each technique

Most bacterial species in a healthy human microbiota belong to the genera Bacteroidetes and Firmicutes (232), whereas Actinobacteria , Proteobacteria , Fusobacteria , and Cyanobacteria , are less abundant (233)

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In addition, they also encode various positive or negative regulatory factors related to pathogenicity, including RBF1, EFG1, and TUP1, as well as genes encoding secreted aspartic acid proteases involved in invading the host endothelial barrier and macrophage phagocytosis (Navarro-Garcia et al., 2001)