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The Journal Of Steroid Biochemistry And Molecular Biology 1986, 25: 127-133

Biomarkers for predicting treatment responses (such as serum 25-hydroxyvitamin D for detecting vitamin D levels) remain underdeveloped, which restricts the formulation of personalized treatment regimens

For instance, Whitehouse et al

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Sample preparation For assays in the head, midgut and abdomen, the tissue from 6 drones was homogenized in lysis buffer [10 mM NaCl, 1% (w/v) Triton X-100, protease inhibitors (2 g.mL 1 antipain, leupeptin and pepstatin A, 25 units.mL 1 aprotinin and 0.1 mg.mL 1 soybean trypsin inhibitor), 40 mM sodium phosphate, pH 7.4] to obtain a 10% (w/v) extract 88