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After the incubation, hECs were washed with Ringer-HEPES buffer (118 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgSO 4 , 5.5 mM d-glucose, 20 mM HEPES, pH 7.4) supplemented with 1% FBS

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However, no further reduction was seen in baseline thermal nociception and acute thermal pain responses (Supplementary Fig

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Proper laboratory technique protects peptide integrity and improves reproducibility